Recombinant Villin (GI-Mucosal & Urogenital Brush Border Marker) Antibody, Clone: [VIL1/7378R], Rabbit, Monoclonal

Catalog Number: NBT-7429-RBM8-P1
Article Name: Recombinant Villin (GI-Mucosal & Urogenital Brush Border Marker) Antibody, Clone: [VIL1/7378R], Rabbit, Monoclonal
Biozol Catalog Number: NBT-7429-RBM8-P1
Supplier Catalog Number: 7429-RBM8-P1
Alternative Catalog Number: NBT-7429-RBM8-P1-100
Manufacturer: NeoBiotechnologies
Host: Rabbit
Category: Antikörper
Application: IHC, WB
Species Reactivity: Human
Immunogen: Recombinant fragment (around aa600-700) of human Villin protein (exact sequence is proprietary)
Alternative Names: VIL1, Villin-1, Villin1
Recognizes a protein of 95kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin.Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.
Clonality: Monoclonal
Clone Designation: [VIL1/7378R]
Molecular Weight: 93kDa
NCBI: 7429
UniProt: P09327
Form: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Western Blot Analysis of A549 cell lysate using Villin Recombinant Rabbit Monoclonal Antibody (VIL1/7378R).
Formalin-fixed, paraffin-embedded human colon adenocarcinoma stained with Villin Recombinant Rabbit Monoclonal Antibody (VIL1/7378R). Inset: PBS instead of primary antibody, secondary only negative control.
Formalin-fixed, paraffin-embedded human colon adenocarcinoma stained with Villin Recombinant Rabbit Monoclonal Antibody (VIL1/7378R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.