This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFPs) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 min
Formalin-fixed, paraffin-embedded hu
Formalin-fixed, paraffin-embedded human Melanoma stained with Vimentin Mouse Monoclonal Antibody (VM1170).
Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Vimentin Mouse Monoclonal Antibody (VM1170).
Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Vimentin Mouse Monoclonal Antibody (VM1170).
Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with Vimentin Mouse Monoclonal Antibody (VM1170).
Formalin-fixed, paraffin-embedded human Uterus stained with Vimentin Mouse Monoclonal Antibody (VM1170).
Formalin-fixed, paraffin-embedded human Ewing&apos,s Sarcoma stained with Vimentin Mouse Monoclonal Antibody (VM1170).
Western Blot Analysis of HEP2 cell lysate using Vimentin Mouse Monoclonal Antibody (VM1170).
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