von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) Antibody, IgG1, Clone: [VWF/4105], Mouse, Monoclonal

Product Overview

• Article Name: von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) Antibody, IgG1, Clone: [VWF/4105], Mouse, Monoclonal
• Biozol Catalog Number: NBT-7450-MSM15-P0
• Supplier Catalog Number: 7450-MSM15-P0
• Alternative Catalog Number: NBT-7450-MSM15-P0-20,NBT-7450-MSM15-P0-100
• Manufacturer: NeoBiotechnologies
• Host: Mouse
• Category: Antikörper
• Application: IHC
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant human vWF fragment spanning around aa 845-949 (exact sequence is proprietary)
• Alternative Names: Coagulation Factor VIII, Factor VIII Related Antigen, F8VWF, von Willebrand Antigen 2, von Willebrand Disease (vWD)

Product Description

von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi s sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [VWF/4105]
• Molecular Weight: 250kDa
• Isotype: IgG1
• NCBI: 7450
• UniProt: P04275
• Form: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Monoclonal Antibody

Application Notes

• Application Notes: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes),Optimal dilutio

Images

Formalin-fixed, paraffin-embedded human uterus stained with vWF Mouse Monoclonal Antibody (VWF/4105).

Formalin-fixed, paraffin-embedded rat lung stained with vWF Mouse Monoclonal Antibody (VWF/4105).

Formalin-fixed, paraffin-embedded mouse lung stained with vWF Mouse Monoclonal Antibody (VWF/4105).

SDS-PAGE Analysis of Purified vWF Mouse Monoclonal Antibody (VWF/4105). Confirmation of Purity and Integrity of Antibody.