Recombinant von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) Antibody, Clone: [VWF/1859R], Rabbit, Monoclonal

Product Overview

• Article Name: Recombinant von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) Antibody, Clone: [VWF/1859R], Rabbit, Monoclonal
• Biozol Catalog Number: NBT-7450-RBM9-P0
• Supplier Catalog Number: 7450-RBM9-P0
• Alternative Catalog Number: NBT-7450-RBM9-P0-20,NBT-7450-RBM9-P0-100
• Manufacturer: NeoBiotechnologies
• Host: Rabbit
• Category: Antikörper
• Application: IHC, WB
• Species Reactivity: Human
• Immunogen: Recombinant fragment of human vWF protein (aa1815-1939) (exact sequence is proprietary)
• Alternative Names: Coagulation Factor VIII, Factor VIII Related Antigen, F8VWF, von Willebrand Antigen 2, von Willebrand Disease (vWD)

Product Description

von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposis sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [VWF/1859R]
• Molecular Weight: 250kDa
• NCBI: 7450
• UniProt: P04275
• Form: 200ug/ml of recombinant MAb Purified by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Recombinant Monoclonal Antibody

Application Notes

• Application Notes: Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 mi

Images

SDS-PAGE Anal

Formalin-fixed, paraffin-embedded human Tonsil stained with vWF Rabbit Recombinant Monoclonal Antibody (VWF/1859R).

Western Blot Analysis A) Recombinant Protein (B) human lung lysate Using Rabbit Recombinant Monoclonal Antibody (VWF/1859R).

SDS-PAGE Analysis of Purified Rabbit Recombinant Monoclonal Antibody (VWF/1859R).