Recombinant N-terminal fragment of human Calnexin protein (around aa 1-300) (exact sequence is proprietary)
Alternative Names:
Calnexin, CANX, CNX, IP90, Major histocompatibility complex class I antigen-binding protein p88, P90
It recognizes a protein of 90kDa, which is identified as Calnexin. Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. It is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA), Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA,
Formalin-f
Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Calnexin Mouse Monoclonal Antibody (CANX/1541).
Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Calnexin Mouse Monoclonal Antibody (CANX/1541).
Formalin-fixed, paraffin-embedded human Small Intestinal Carcinoma stained with Calnexin Mouse Monoclonal Antibody (CANX/1541).
Confocal immunofluorescence image of HeLa cells using Calnexin Mouse Monoclonal Antibody (CANX/1541), labeled in Green. Reddot is used to label the nuclei Red.
Western Blot Analysis of Human Kidney lysate using Calnexin Mouse Monoclonal Antibody (CANX/1541).
Western Blot Analysis of HeLa and MCF-7 cell lysate using Calnexin Mouse Monoclonal Antibody (CANX/1541).
SDS-PAGE Analysis Purified Calnexin Mouse Monoclonal Antibody (CANX/1541). Confirmation of Integrity and Purity of Antibody.
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