Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. It is found on all mature T-lymphocytes, NK, T-cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. It is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly Intracytoplasmic. This MAb has been reported to have potent immunosuppressive properties in vivo and has been proven effective in the treatment of renal, heart and liver allograft rejection.
200ug/ml of Ab purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Flow Cytometry (0.5-1ug/million cells), ,Immunohistochemistry (acetone fixed frozen sections), ,Immunofluorescence (1-2ug/ml), ,Functional Studies (Order Ab without Azide),,Optimal dilution for a specific application should be determined.
SDS-PAGE Analysis of Purified CD3e Mouse Monoclonal Antibody (OKT3). Confirmation of Purity and Integrity of Antibody.
Flow cytometry of lymphocyte gated PBMCs stained with CD3 mouse monoclonal antibody (OKT3) (red) or isotype control (gray) followed by goat anti-mouse CF640R (red)
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