Recombinant CD4 (T-Helper/Inducer Cell Marker) Antibody, IgG2a, Clone: [rCD4/3930], Mouse, Monoclonal

Product Overview

• Article Name: Recombinant CD4 (T-Helper/Inducer Cell Marker) Antibody, IgG2a, Clone: [rCD4/3930], Mouse, Monoclonal
• Biozol Catalog Number: NBT-920-MSM10-P1ABX
• Supplier Catalog Number: 920-MSM10-P1ABX
• Alternative Catalog Number: NBT-920-MSM10-P1ABX-100
• Manufacturer: NeoBiotechnologies
• Host: Mouse
• Category: Antikörper
• Application: IHC, WB
• Species Reactivity: Human
• Immunogen: Recombinant fragment (around aa 200-300) of human CD4 protein (exact sequence is proprietary)
• Alternative Names: L3T4, Leu3, Ly-4, Lymphocyte antigen CD4, p55, T cell antigen T4/LEU3, T cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

Product Description

Recognizes a protein of 55kDa, identified as CD4. It is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigens and is also a receptor for the human immunodeficiency virus. This protein is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. The majority of peripheral T-cell lymphomas are derived from the T-helper/regulatory cell subset so that most mature T-cell neoplasms are CD4+/CD8-. Anti-CD4 is used in the immunohistochemical staining of lymphoproliferative disorders to evaluate tumors with CD4 aberrant expression.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [rCD4/3930]
• Molecular Weight: 55kDa
• Isotype: IgG2a
• NCBI: 920
• UniProt: P01730
• Form: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Recombinant Monoclonal Antibody

Application Notes

• Application Notes: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal diluti

Images

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negat

Formalin-fixed, paraffin-embedded human lymph node stained with CD4 Recombinant Mouse Monoclonal Antibody (rCD4/3930). HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using rCD4/3930 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human tonsil stained with CD4 Recombinant Mouse Monoclonal Antibody (rCD4/3930).

SDS-PAGE Analysis Purified CD4 Recombinant Mouse Monoclonal Antibody (rCD4/3930). Confirmation of Integrity and Purity of Antibody.

Formalin-fixed, paraffin-embedded human lymph node stained with CD4 Recombinant Mouse Monoclonal Antibody (rCD4/3930).

Western Blot Analysis of human tonsil tissue lysate using CD4 Recombinant Mouse Monoclonal Antibody (rCD4/3930).