Recombinant Aurora B (Proliferation Marker) Antibody, Clone: [AURKB/3121R], Rabbit, Monoclonal

Product Overview

• Article Name: Recombinant Aurora B (Proliferation Marker) Antibody, Clone: [AURKB/3121R], Rabbit, Monoclonal
• Biozol Catalog Number: NBT-9212-RBM6-P1ABX
• Supplier Catalog Number: 9212-RBM6-P1ABX
• Alternative Catalog Number: NBT-9212-RBM6-P1ABX-100
• Manufacturer: NeoBiotechnologies
• Host: Rabbit
• Category: Antikörper
• Application: IHC, WB
• Species Reactivity: Human
• Immunogen: Recombinant fragment (around aa 89-251) of human Aurora B protein (exact sequence is proprietary)
• Alternative Names: AIK2, AIM-1, ARK-2, AurB, AURKB, Aurora-1, Aurora and Ipl1 like midbody associated protein 1, Aurora kinase B, Aurora-B, Aurora-related kinase 2, Aurora/IPL1-related kinase 2, IPL1, Protein phosphatase 1 regulatory subunit 48 (PPP1R48), Serine/threonine-protein kinase 12, Serine/threonine-protein kinase aurora-B, STK1, STK12, STK5

Product Description

Recognizes a protein of 39kDa, which is identified as Aurora B. The serine/threonine protein kinase aurora B (Aurora B) is a chromosomal passenger protein critical for accurate chromosome segregation, cytokinesis, protein localization to the centromere and kinetochore, correct microtubule-kinetochore attachment, and regulation of the mitotic checkpoint. Aurora B forms a tight complex with inner centrosome protein and survivin. Inactivation of any of these proteins causes similar defects in chromosome segregation. A significant overexpression of Aurora B has been found in a variety of human tumors including non-small cell lung carcinoma, astrocytoma, seminoma and carcinomas of the colon, prostate, endometrium and thyroid. The expression level of Aurora B is associated with cell proliferation and prognosis in these tumors.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [AURKB/3121R]
• Molecular Weight: 39kDa
• NCBI: 9212
• UniProt: Q96GD4
• Form: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Recombinant Monoclonal Antibody

Application Notes

• Application Notes: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes), Optimal dilution f

Images

Formalin-fixed, paraffin-embedded human Colon stained with Aurora B Rabbit Recombinant Monoclonal Antibody (AURKB/3121R).

SDS-PAGE Analysis of Purified Aurora B Rabbit Recombinant Monoclonal (AURKB/3121R). Confirmation of Purity and Integrity of Antibody.

Western blot analysis of Human Liver, Mouse Liver, Rat Liver, Hamster Liver and Guinea pig Liver tissue lysates using Aurora B Rabbit Recombinant Monoclonal Antibody (AURKB/3121R).