The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that serves as a coreceptor for TCR recognition of MHC class I associated peptides and supports CTL activation by binding to the MHC, while making no direct contact with the peptide.CD8 is expressed on cytotoxic suppressor T cells. It is expressed as a heterodimer of CD8(TM) and CD8A°a. CD8 is expressed on approximately one-third of peripheral blood T cells (the CD4-negative cells). CD8 has also been detected at a low level on some natural killer (NK) cells. In normal human tonsil, large numbers of CD8+ lymphocytes were present within the paracortex, occasionally positive cells were also identified within germinal centers and within the investing squamous epithelium. In other tissues, only lymphoid cells and cells of histiocyte lineage showed positive staining for CD8.
200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Application Notes:
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilutio
IHC analysis of
Formalin-fixed, paraffin-embedded human lymph node stained with CD8a Recombinant Rabbit Monoclonal Antibody (CD8/4391R).
Formalin-fixed, paraffin-embedded human tonsil stained with CD8a Recombinant Rabbit Monoclonal Antibody (CD8/4391R).
IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using CD8/4391R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.
Formalin-fixed, paraffin-embedded human tonsil stained with CD8a Recombinant Rabbit Monoclonal Antibody (CD8/4391R).
SDS-PAGE Analysis Purified CD8a Recombinant Rabbit Monoclonal Antibody (CD8/4391R). Confirmation of Purity and Integrity of Antibody.
Formalin-fixed, paraffin-embedded human lymph node stained with CD8a Recombinant Rabbit Monoclonal Antibody (CD8/4391R).
Analysis of Protein Array containing more than 19,000 full-length human proteins using CD8a Recombinant Rabbit Monoclonal Antibody (CD8/4391R). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD&apos,s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD&apos,s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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