APY, ATOPY, B-lymphocyte cell-surface antigen B1, Bp35, Fc epsilon receptor I beta chain, Fc Fragment of IgE high affinity I receptor for beta polypeptide, FCER1B, High affinity immunoglobulin epsilon receptor subunit beta, IgE Fc receptor subunit beta, IGEL, IGER, IGHER, LEU16, Leukocyte surface antigen Leu-16, Ly44, Membrane spanning 4 domains subfamily A member 2, Membrane-spanning 4-domains subfamily A member 1 (MS4A1)
Recognizes a protein of 30-33kDa, which is identified as CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre-B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.
200ug/ml of recombinant MAb purified by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Flow Cytometric An
Formalin-fixed, paraffin-embedded human tonsil stained with CD20 Mouse Recombinant Monoclonal Antibody (rIGEL/773).
Formalin-fixed, paraffin-embedded human lymph node stained with CD20 Mouse Recombinant Monoclonal Antibody (rIGEL/773).
Flow Cytometric Analysis of Raji cells. CD20 Recombinant Mouse Monoclonal Antibody (rIGEL/773) followed bygoat anti-mouse IgG-CF488 (blue), isotype control (red).
SDS-PAGE Analysis PurifiedCD20 Mouse Recombinant Monoclonal Antibody (rIGEL/773). Confirmation of Purity and Integrity of Antibody.
Immunofluorescence staining of Raji cells using CD20 Mouse Recombinant Monoclonal Antibody (rIGEL/773) followed by goat anti-mouse IgG-CF488 (green). Nuclei stained with RedDot.
Analysis of Protein Array containing more than 19,000 full-length human proteins using CD20 Monospecific Recombinant Mouse Monoclonal Antibody (rIGEL/773). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD&apos,s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD&apos,s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Western Blot Analysis of Raji cell lysate using CD20 Mouse Monoclonal Antibody (rIGEL/773).
Western Blot Analysis of Raji cell lysate using CD20 Recombinant Mouse Monoclonal Antibody (rIGEL/773).
Western Blot Analysis of Human Intestine and Human Spleen tissue lysates using CD20 Mouse Monoclonal Antibody (rIGEL/773).
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