Recombinant fragment (around aa 97-297) corresponding to C-terminal of human CD20 protein (exact sequence is proprietary)
Alternative Names:
APY, ATOPY, B-lymphocyte cell-surface antigen B1, Bp35, Fc epsilon receptor I beta chain, Fc Fragment of IgE high affinity I receptor for beta polypeptide, FCER1B, High affinity immunoglobulin epsilon receptor subunit beta, IgE Fc receptor subunit beta, IGEL, IGER, IGHER, LEU16, Leukocyte surface antigen Leu-16, Ly44, MS4A1, MS4A2
Recognizes a protein of 30-33kDa, which is identified as CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.
200ug/ml of Ab purified by Protein A Column. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Western Blot Analysis of human tonsil tissue lysate using CD20 Recombinant Rabbit Monoclonal Antibody (IGEL/6993R).
Western Blot Analysis of Ramos cell lysate using CD20 Recombinant Rabbit Monoclonal Antibody (IGEL/6993R).
Western Blot Analysis of human Raji lysate using CD20 Recombinant Rabbit Monoclonal Antibody (IGEL/6993R).
Western Blot Analysis of Raji cell lysate using CD20 Recombinant Rabbit Monoclonal Antibody (IGEL/6993R).
SDS-PAGE Analysis of Purified CD20 Recombinant Rabbit Monoclonal Antibody (IGEL/6993R). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of Raji and Ramos cell lysates using CD20 Recombinant Rabbit Monoclonal Antibody (IGEL/6993R).
Western blot analysis of Raji cell lysate using CD20 Recombinant Rabbit Monoclonal Antibody (IGEL/6993R).
