Recognizes a protein of 130-140kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA),Flow Cytometry (1-2ug/million cells),Western Blot (1-2ug/ml),Immunofluorescence (1-2ug/ml),Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tiss
Western Blot Analy
Formalin-fixed, paraffin-embedded human Spleen stained with CD22-Monospecific Mouse Monoclonal Antibody (BLCAM/1795).
Formalin-fixed, paraffin-embedded human Tonsil stained with CD22-Monospecific Mouse Monoclonal Antibody (BLCAM/1795).
Western Blot Analysis of Raji cell lysate using CD22-Monospecific Mouse Monoclonal Antibody (BLCAM/1795).
Western Blot Analysis of Raji and Ramos cell lysates using CD22-Monospecific Mouse Monoclonal Antibody (BLCAM/1795).
SDS-PAGE Analysis Purified CD22-Monospecific Mouse Monoclonal Antibody (BLCAM/1795). Confirmation of Purity and Integrity of Antibody.
Flow Cytometric Analysis of paraformaldehyde-fixed MOLT4 cells. CD22-Monospecific Mouse Monoclonal Antibody (BLCAM/1795) followed by goat anti-Mouse IgG-CF488 (Blue), Isotype Control (Red).
Immunofluorescent staining of paraformaldehyde-fixed Ramos cells using CD22-Monospecific Mouse Monoclonal Antibody (BLCAM/1795) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.
Analysis of Protein Array containing more than 19,000 full-length human proteins using CD22-Monospecific Mouse Monoclonal Antibody (BLCAM/1795) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD&apos,s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD&apos,s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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