Recombinant CD22 / BL-CAM (B-Cell Marker) Antibody, Clone: [BLCAM/8113R], Rabbit, Monoclonal

Product Overview

• Article Name: Recombinant CD22 / BL-CAM (B-Cell Marker) Antibody, Clone: [BLCAM/8113R], Rabbit, Monoclonal
• Biozol Catalog Number: NBT-933-RBM10-P1
• Supplier Catalog Number: 933-RBM10-P1
• Alternative Catalog Number: NBT-933-RBM10-P1-100
• Manufacturer: NeoBiotechnologies
• Host: Rabbit
• Category: Antikörper
• Application: IHC, WB
• Species Reactivity: Human
• Immunogen: Recombinant fragment (around aa1-200) of human BL-CAM/CD22 protein (exact sequence is proprietary)
• Alternative Names: B-lymphocyte cell adhesion molecule (BL-CAM), B-cell receptor CD22, CD22, Lectin 2, Lyb8, Sialic acid-binding Ig-like lectin 2 (Siglec-2), SIGLEC2, T-cell surface antigen Leu-14

Product Description

Recognizes a protein of 130-140kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [BLCAM/8113R]
• Molecular Weight: 130-140kDa
• NCBI: 933
• UniProt: P20273
• Form: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Images

SDS-PAGE Analysis of Purified CD22 Recombinant Rabbit Monoclonal Antibody(BLCAM/8113R). Confirmation of Purity and Integrity of Antibody

Western Blot Analysis of Human Spleen, Raji and Ramos lysates using CD22 Recombinant Rabbit Monoclonal Antibody (BLCAM/8113R).

Western Blot Analysis of Human Spleen, Mouse Spleen, Rat Spleen, Hamster Spleen, Guinea pig Spleen and Rabbit Spleen tissue lysates using CD22 Recombinant Rabbit Monoclonal Antibody (BLCAM/8113R).

Formalin-fixed, paraffin-embedded human tonsil stained with CD22 Recombinant Rabbit Monoclonal Antibody (BLCAM/8113R). Inset: PBS instead of primary antibody, secondary control.