Recognizes a protein of a disulfide-linked 120kDa dimer, identified as CD27. It is expressed on the majority of peripheral T cells, medullary thymocytes, memory-type B cells, and natural killer cells. It is a transmembrane phosphoglycoprotein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD27 binds to its ligand CD70, a member of the TNF family, and induces T-cell co-stimulation and B-cell activation. It also interacts with TRAFs and is involved in activation of NFkB and SAPK/JNK and induces apoptosis.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells),,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by
Formalin-fixed, paraffin-embedded human Tonsil stained with CD27 Recombinant Mouse Monoclonal Antibody (rLPFS2/1611).
SDS-PAGE Analysis of Purified CD27 Recombinant Mouse Monoclonal Antibody (rLPFS2/1611). Confirmation of Integrity and Purity of Antibody.
Flow Cytometric Analysis of Ramos cells using CD27 Recombinant Mouse Monoclonal Antibody (rLPFS2/1611) followed by goat anti-Mouse IgG-CF488 (Blue), Isotype Control (Red).
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