This antibody recognizes a transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels 85% of angiosarcoma and Kaposis sarcoma, but shows low specificity.
200ug/ml of recombinant MAb Purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at R
F
Formalin-fixed, paraffin-embedded human Angiosarcoma stained with CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R).
Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R).
Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R).
Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R).
Formalin-fixed, paraffin-embedded human Tonsil stained with CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R).
SDS-PAGE Analysis Purified CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R). Confirmation of Integrity and Purity of Antibody.
Flow Cytometric Analysis of human Jurkat cells using CD34 CD34 Recombinant Rabbit MAb (HPCA1/2598R) followed by Goat anti-Mouse IgG-CF488 (Blue), Isotype Control (Red).
SDS-PAGE Analysis Purified CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R). Confirmation of Integrity and Purity of Antibody.
Western Blot Analysis of Human Testis tissue lysate using CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R).
Western blot analysis of Human Testis, Mouse Testis, Rat Testis, Hamster Testis, Guinea pig Testis and Rabbit Testis tissue lysate using CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R).
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