Recombinant fragment of human ATG5 protein (around aa 1-119) (exact sequence is proprietary)
Alternative Names:
APG5 autophagy 5 like (APG5L), Apoptosis-specific protein (ASP), ATG5, Autophagy related 5, hAPG5
The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. The ATG5 protein is essential for autophagy, a process that is usually beneficial for cells to self-degrade their own components when they are no longer useful.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
ELISA (For coating use Ab at 1-5ug/ml, order Ab without BSA), Western Blot (1-2ug/ml),,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9
Formalin-fixed, paraffin-embedded h
Formalin-fixed, paraffin-embedded human Brain stained with ATG5 Mouse Monoclonal Antibody (ATG5/2553).
Western Blot Analysis of THP-1 cell and human Brain tissue lysate using ATG5 Mouse Monoclonal Antibody (ATG5/2553).
Formalin-fixed, paraffin-embedded human Uterus stained with ATG5 Mouse Monoclonal Antibody (ATG5/2553).
Formalin-fixed, paraffin-embedded human Endometrium stained with ATG5 Mouse Monoclonal Antibody (ATG5/2553).
Western Blot Analysis of K562, U-87, A431 cell lysates using ATG5 Mouse Monoclonal Antibody (ATG5/2553).
SDS-PAGE Analysis of Purified ATG5 Mouse Monoclonal Antibody (ATG5/2553). Confirmation of Purity and Integrity of Antibody.
Western blot analysis of Human Liver, Mouse Liver, Rat Liver, Hamster Liver, Guinea pig Liver and Rabbit Liver tissue lysates using ATG5 Mouse Monoclonal Antibody (ATG5/2553).
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