CD44 / HCAM (Cancer Stem Cell Marker) Antibody, IgG1, Clone: [DF1485], Mouse, Monoclonal

Product Overview

• Article Name: CD44 / HCAM (Cancer Stem Cell Marker) Antibody, IgG1, Clone: [DF1485], Mouse, Monoclonal
• Biozol Catalog Number: NBT-960-MSM3-P0
• Supplier Catalog Number: 960-MSM3-P0
• Alternative Catalog Number: NBT-960-MSM3-P0-20,NBT-960-MSM3-P0-100
• Manufacturer: NeoBiotechnologies
• Host: Mouse
• Category: Antikörper
• Application: FC, IF, IHC
• Species Reactivity: Human, Mouse
• Immunogen: Purified CD44 antigen (PGp-1) from lymphocyte membrane
• Alternative Names: LHR, BA-1, chondroitin sulfate proteoglycan 8 (CSPG8), Epican, Extracellular Matrix Receptor III (ECM III), GP90 Lymphocyte Homing Adhesion Receptor, HCAM, HCELL, hematopoietic cell E- and L-selectin ligand, Heparan Sulfate Proteoglycan, Hermes Antigen, HAS, HUTCH I, Hyaluronate Receptor, Indian blood group, Inlu Related p80 Glycoprotein, Ly 24, MDU2, MDU3, MIC4, MUTCH I, Phagocytic Glycoprotein 1 (PGP-1)

Product Description

Recognizes a cell surface glycoprotein of 80-95kDa (CD44) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [DF1485]
• Molecular Weight: 80-95kDa
• Isotype: IgG1
• NCBI: 960
• UniProt: P16070
• Form: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Monoclonal Antibody

Application Notes

• Application Notes: Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 mi

Images

Formalin-fixed, paraffin-embedded human Breast tissue stained with CD44 Mouse Monoclonal Antibody (DF1485).

Confocal immunofluorescence image HeLa cells using CD44 Mouse Monoclonal Antibody (DF1485) labeled Green (CF488) and Reddot is used to label the nuclei Red.

SDS-PAGE Analysis of Purified CD44 Mouse Monoclonal Antibody (DF1485). Confirmation of Purity and Integrity of Antibody.