LHR, BA-1, chondroitin sulfate proteoglycan 8 (CSPG8), Epican, Extracellular Matrix Receptor III (ECM III), GP90 Lymphocyte Homing Adhesion Receptor, HCAM, HCELL, hematopoietic cell E- and L-selectin ligand, Heparan Sulfate Proteoglycan, Hermes Antigen, HAS, HUTCH I, Hyaluronate Receptor, Indian blood group, Inlu Related p80 Glycoprotein, Ly 24, MDU2, MDU3, MIC4, MUTCH I, Phagocytic Glycoprotein 1 (PGP-1)
Recognizes a cell surface glycoprotein of 80-95kDa (CD44) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. This MAb selectively interferes with lymphocyte binding to lymph node, mucosal and synovial endothelium. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Application Notes:
Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 2
Formalin-fixed, paraffin-embedded human Lymph Node stained with CD44 Rabbit Recombinant Monoclonal Antibody (HCAM/2875R).
SDS-PAGE Analysis of Purified CD44 Rabbit Recombinant Monoclonal Antibody (HCAM/2875R). Confirmation of Integrity and Purity of Antibody.
Western Blot Analysis of HeLa cell lysate using CD44 Rabbit Recombinant Monoclonal Antibody (HCAM/2875R).
Western Blot Analysis of MCF7 lysate using CD44 Rabbit Recombinant Monoclonal Antibody (HCAM/2875R)
Western Blot Analysis of Human Lung, Human Pancreas, A549, and HeLa lysates using CD44 Recombinant Rabbit Monoclonal Antibody (HCAM/2875R).
Western Blot Analysis of Human Lung, Colon, Small Intestine, Tonsil tissue lysate using CD44 Recombinant Rabbit Monoclonal Antibody (HCAM/2875R).
Western Blot Analysis of MDA-MB-231 lysate using CD44 Rabbit Recombinant Monoclonal Antibody (1HCAM/2875R)
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