CD63 (Late Endosomes Marker) Antibody, IgG1, Clone: [NKI/C3], Mouse, Monoclonal

Article Name:	CD63 (Late Endosomes Marker) Antibody, IgG1, Clone: [NKI/C3], Mouse, Monoclonal
Biozol Catalog Number:	NBT-967-MSM1-P1ABX
Supplier Catalog Number:	967-MSM1-P1ABX
Alternative Catalog Number:	NBT-967-MSM1-P1ABX-100
Manufacturer:	NeoBiotechnologies
Host:	Mouse
Category:	Antikörper
Application:	FC, IF, IHC
Species Reactivity:	Human, Mouse
Immunogen:	Smooth plasma membrane fraction of MeWo cells
Alternative Names:	gp55, granulophysin, Lysosomal-associated membrane protein 3 (LAMP-3), Mast cell antigen AD1, melanoma 1 antigen, Melanoma-associated antigen MLA1, Melanoma-associated antigen ME491, MLA1, NGA, Ocular melanoma-associated antigen, OMA81H, PTLGP40, Tetraspanin-30, TSPAN30

This MAb recognizes protein of 26kDa-60kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

Clonality:	Monoclonal
Clone Designation:	[NKI/C3]
Molecular Weight:	26kDa (core protein), 30-60kDa (glycosylated)
Isotype:	IgG1
NCBI:	967
UniProt:	P08962
Form:	200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:	Monoclonal Antibody

Application Notes:

Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-

	Flow Cytometric Anal
	Flow cytometric analysis of human CD63 on MCF-7 cells. Black: cells alone, Green: isotype control, Red: PE-labeled CD63 Mouse Monoclonal Antibody (NKI/C3).
	Immunofluorescent Analysis of HeLa cells using CF488-labeled CD63 Mouse Monoclonal Antibody (NKI/C3). F-actin filaments are labeled with Dylight 554 phalloidin (red). DAPI was used to stain the cell nucleus (blue).
	Formalin-fixed, paraffin-embedded human melanoma stained with CD63 Mouse Monoclonal Antibody (NKI/C3) (AEC Chromogen)
	Formalin-fixed, paraffin-embedded mouse spleen stained with CD63 Mouse Monoclonal Antibody (NKI/C3) (DAB Chromogen)
	Flow Cytometric Analysis of PFA-fixed U87MG cells. CD63 Mouse Monoclonal Antibody (NKI/C3) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).
	SDS-PAGE Analysis Purified CD63 Mouse Monoclonal Antibody (NKI/C3). Confirmation of Purity and Integrity of Antibody.
	Flow cytometric analysis of human CD63 on MCF-7 cells. Black: cells alone, Green: isotype control, Red: PE-labeled CD63 Mouse Monoclonal Antibody (NKI/C3).
	Flow cytometry analysis of live MCF-7 cells. Unstained (gray) or stained with biotin-labeled CD63 monoclonal antibody (NKI-C3) followed by streptavidin-CF568 (orange).
	Flow cytometric analysis of human CD63 on MCF-7 cells. Black: cells alone, Green: isotype control, Red: PE-labeled CD63 Mouse Monoclonal Antibody (NKI/C3).
	Flow Cytometric Analysis of PFA-fixed U87MG cells. CD63 Mouse Monoclonal Antibody (NKI/C3) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).