CD63 (Late Endosomes Marker) Antibody, IgG1, Clone: [MX-49.129.5], Mouse, Monoclonal

Article Name:	CD63 (Late Endosomes Marker) Antibody, IgG1, Clone: [MX-49.129.5], Mouse, Monoclonal
Biozol Catalog Number:	NBT-967-MSM2-P0
Supplier Catalog Number:	967-MSM2-P0
Alternative Catalog Number:	NBT-967-MSM2-P0-20,NBT-967-MSM2-P0-100
Manufacturer:	NeoBiotechnologies
Host:	Mouse
Category:	Antikörper
Application:	FC, IF, IHC, WB
Species Reactivity:	Human
Immunogen:	Full length CD63 of human origin
Alternative Names:	gp55, granulophysin, Lysosomal-associated membrane protein 3 (LAMP-3), Mast cell antigen AD1, Melanoma-associated antigen MLA1, Melanoma-associated antigen ME491, NGA, Ocular melanoma-associated antigen, OMA81H, Tetraspanin-30, TSPAN30

This MAb recognizes protein of 26kDa-60kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

Clonality:	Monoclonal
Clone Designation:	[MX-49.129.5]
Molecular Weight:	26kDa (core protein), 30-60kDa (glycosylated)
Isotype:	IgG1
NCBI:	967
UniProt:	P08962
Form:	200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:	Monoclonal Antibody

Application Notes:

Flow Cytometry (0.5-1ug/million cells), Immunofluorescence (0.5-1ug/ml), Western Blot (0.5-1ug/ml),,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris wi

	Immunofluorescence Analysis of HeLa cell
	Flow Cytometry of human CD63 on MCF-7 cells.Black: Cells alone, Grey: Isotype Control, Green: CF488-labeled CD63 Mouse Monoclonal Antibody (MX-49.129.5).
	Immunofluorescence Analysis of HeLa cells stained using CF488-labeled-CD63 Monoclonal Antibody (MX-49.129.5) (green). F-actin filaments labeled with phalloidin (red). Nuclei stained with DAPI (blue).
	Formalin-fixed, paraffin-embedded human melanoma stained with CD63 Mouse Monoclonal Antibody (MX-49.129.5).
	Flow cytometric analysis of bead-bound exosomes derived from MCF-7 cells. CD63 Mouse Monoclonal Antibody (MX-49.129.5) followed by goat anti-mouse IgG-CF568 (orange), unstained exosomes (gray).
	Western blot analysis of human spleen tissue lysate using CD63 Mouse Monoclonal Antibody (MX-49.129.5).
	Formalin-fixed, paraffin-embedded mouse spleen stained with CD63 Mouse Monoclonal Antibody (MX-49.129.5).
	Immunofluorescence Analysis of HeLa cells stained using CF488-labeled-CD63 Monoclonal Antibody (MX-49.129.5) (green). F-actin filaments labeled with phalloidin (red). Nuclei stained with DAPI (blue).
	Immunofluorescence Analysis of PFA-fixed U87MG cells stained using CD63 Mouse Monoclonal Antibody (MX-49.129.5) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).
	Flow cytometric analysis of NIH/3T3 cells. Black: Cells alone, Green: Isotype Control, Red: PE-labeled CD63 Mouse Monoclonal Antibody (MX-49.129.5).
	Formalin-fixed, paraffin-embedded human prostate carcinoma stained with CD63 Mouse Monoclonal Antibody (MX-49.129.5).
	Flow cytometric analysis of MCF-7 cells. CD63 Mouse Monoclonal Antibody (MX-49.129.5) followed by goat anti-mouse IgG-CF488 (green), unstained cells (gray).
	Flow cytometric analysis of bead-bound exosomes derived from MCF-7 cells. CD63 Mouse Monoclonal Antibody (MX-49.129.5) followed by goat anti-mouse IgG-CF488 (green), unstained exosomes (gray).
	Flow cytometric analysis of MCF-7 cells. CD63 Mouse Monoclonal Antibody (MX-49.129.5) followed by goat anti-mouse IgG-CF568 (orange), unstained cells (gray).
	Flow cytometric analysis of NIH/3T3 cells. Black: Cells alone, Green: Isotype Control, Red: PE-labeled CD63 Mouse Monoclonal Antibody (MX-49.129.5).
	Flow Cytometric staining of CD63 on human PBMC cells. Black: Cells alone, Green: Isotype Control, Red: PE-labeled CD63 Mouse Monoclonal Antibody (MX-49.129.5).