Recombinant CD63 (Late Endosomes Marker) Antibody, Clone: [LAMP3/8799R], Rabbit, Monoclonal

Product Overview

• Article Name: Recombinant CD63 (Late Endosomes Marker) Antibody, Clone: [LAMP3/8799R], Rabbit, Monoclonal
• Biozol Catalog Number: NBT-967-RBM27-P0
• Supplier Catalog Number: 967-RBM27-P0
• Alternative Catalog Number: NBT-967-RBM27-P0-20,NBT-967-RBM27-P0-100
• Manufacturer: NeoBiotechnologies
• Host: Rabbit
• Category: Antikörper
• Application: IHC, WB
• Species Reactivity: Human
• Immunogen: Recombinant full-length human CD63 protein
• Alternative Names: gp55, granulophysin, Lysosomal-associated membrane protein 3 (LAMP-3), Mast cell antigen AD1, melanoma 1 antigen, Melanoma-associated antigen MLA1, Melanoma-associated antigen ME491, MLA1, NGA, Ocular melanoma-associated antigen, OMA81H, PTLGP40, Tetraspanin-30, TSPAN30

Product Description

This MAb recognizes protein of 26kDa-60kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [LAMP3/8799R]
• Molecular Weight: 26kDa (core protein), 30-60kDa (glycosylated)
• NCBI: 967
• UniProt: P08962
• Form: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Recombinant Monoclonal Antibody

Application Notes

• Application Notes: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes),Optimal dilution f

Images

Western Blot Analysis of Brain, Lung and Pancreas tissue lysates of different species using CD63 Recombinant Rabbit Monoclonal Antibody (LAMP3/8799R).

Western Blot Analysis of Human Brain, Human Heart, Human Kidney, Human Lung and Human Pancreas tissue lysates using CD63 Recombinant Rabbit Monoclonal Antibody (LAMP3/8799R).

Formalin-fixed, paraffin-embedded human tonsil stained with CD63 Recombinant Rabbit Monoclonal Antibody (LAMP3/8799R). Inset: PBS instead of primary antibody, secondary only negative control.

SDS-PAGE Analysis of Purified CD63 Recombinant Rabbit Monoclonal Antibody (LAMP3/8799R). Confirmation of Purity and Integrity of Antibody.