CD74 (B-Cell Marker) Antibody, IgG1, Clone: [LN-2], Mouse, Monoclonal

Product Overview

• Article Name: CD74 (B-Cell Marker) Antibody, IgG1, Clone: [LN-2], Mouse, Monoclonal
• Biozol Catalog Number: NBT-972-MSM1-P1
• Supplier Catalog Number: 972-MSM1-P1
• Alternative Catalog Number: NBT-972-MSM1-P1-100
• Manufacturer: NeoBiotechnologies
• Host: Mouse
• Category: Antikörper
• Application: FC, IF, IHC, WB
• Species Reactivity: Human, Mouse, Primate
• Immunogen: SU-DHL-4 lymphoma cells
• Alternative Names: CLIP, DHLAG, Gamma chain of class II antigens, HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, HLADR-gamma (HLADG), Ia antigen-associated invariant chain, la-gamma, Major histocompatibility complex class II invariant chain, MHC HLA-DR gamma chain

Product Description

It recognizes a protein of ~35kDa, identified as CD74 (Workshop IV). CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).

Product Properties

• Clonality: Monoclonal
• Clone Designation: [LN-2]
• Molecular Weight: 33-41kDa
• Isotype: IgG1
• NCBI: 972
• UniProt: P04423
• Form: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Images

Formalin-fixed, paraffin-embedded human Tonsil stained with CD74 Mouse Monoclonal Antibody (LN-2).

Formalin-fixed, paraffin-embedded human Spleen stained with CD74 Mouse Monoclonal Antibody (LN-2).

Immunofluorescence Analysis of Raji cells labeling CD74

Formalin-fixed, paraffin-embedded human Tonsil stained with CD74 Mouse Monoclonal Antibody (LN-2).

Formalin-fixed, paraffin-embedded human Spleen stained with CD74 Mouse Monoclonal Antibody (LN-2).

Immunofluorescence Analysis of Raji cells labeling CD74 with CD74 Mouse Monoclonal Antibody (LN-2) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red)

Flow Cytometric Analysis of Human Raji cells using CD74 Mouse Monoclonal Antibody (LN-2) followed by Goat anti-Mouse IgG-CF488 (Blue). Isotype Control (Red).

Western blot analysis of Raji cell lysate using CD74 Mouse Monoclonal Antibody (LN-2).

SDS-PAGE Analysis of Purified CD74 Mouse Monoclonal Antibody (LN-2). Confirmation of Purity and Integrity of Antibody.