Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This MAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.
Clonality:
Monoclonal
Clone Designation:
[SPM102]
Molecular Weight:
Depends upon the phosphorylated target
Isotype:
IgG2b
NCBI:
Not Applicable
Form:
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), Immunoprecipitation (1-2ug/500ug protein lysate), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (No pretreatment is required for stainin
Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Phosphotyrosine Monoclonal Antibody (SPM102).
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