Cytokeratin, pan (Epithelial Marker) Antibody, IgG1, Clone: [AE-1/AE-3], Mouse, Monoclonal

Product Overview

• Article Name: Cytokeratin, pan (Epithelial Marker) Antibody, IgG1, Clone: [AE-1/AE-3], Mouse, Monoclonal
• Biozol Catalog Number: NBT-MSM2-371-P0
• Supplier Catalog Number: MSM2-371-P0
• Alternative Catalog Number: NBT-MSM2-371-P0-20,NBT-MSM2-371-P0-100
• Manufacturer: NeoBiotechnologies
• Host: Mouse
• Category: Antikörper
• Application: FC, IF, IHC, WB
• Species Reactivity: Human
• Immunogen: Human epidermal keratin
• Alternative Names: K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39

Product Description

Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67kDa (CK1), 64kDa (CK3), 59kDa (CK4), 58kDa (CK5), 56kDa (CK6), 52kDa (CK8), 56.5kDa (CK10), 50kDa (CK14), 50kDa (CK15), 48kDa (CK16), 40kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [AE-1/AE-3]
• Molecular Weight: 40-67kDa
• Isotype: IgG1
• NCBI: 374454
• UniProt: Q7Z794
• Form: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Monoclonal Antibody

Application Notes

• Application Notes: Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml for 2 hours at RT), Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in

Images

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with pan Cytokeratin Monoclonal Antibody cocktail (AE-1/AE3).

Flow Cytometric analysis of human Pan Cytokeratin on HeLa cells. Black: cells alone, Green: Isotype Control, Red: PE-labeled Pan-Cytokeratin Monoclonal Antibody (AE-1/AE-3).