Human Nucleolar Antigen (Marker For Human Cells) Antibody, IgG1, Clone: [NM95], Mouse, Monoclonal

Product Overview

• Article Name: Human Nucleolar Antigen (Marker For Human Cells) Antibody, IgG1, Clone: [NM95], Mouse, Monoclonal
• Biozol Catalog Number: NBT-MSM2-95-P1ABX
• Supplier Catalog Number: MSM2-95-P1ABX
• Alternative Catalog Number: NBT-MSM2-95-P1ABX-100
• Manufacturer: NeoBiotechnologies
• Host: Mouse
• Category: Antikörper
• Application: FC, IF, IHC
• Species Reactivity: Human
• Immunogen: Nuclei of myeloid leukemia biopsy cells
• Alternative Names: Not Known

Product Description

This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb NM95 recognizes an antigen associated with the nucleoli in human cells. It can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [NM95]
• Molecular Weight: Not Known
• Isotype: IgG1
• NCBI: Not Known
• Form: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Monoclonal Antibody

Application Notes

• Application Notes: Flow Cytometry (1-2ug/million cells). ,Immunofluorescence (1-2ug/ml), ,Immunocytochemistry (Acetone-fixed cells) (1-2ug/ml for 30 minutes at RT),,Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissu

Images

Formalin-fixed, paraffin-embedded hum

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CF488 Conjugate of Nucleolar Mouse Monoclonal Antibody (NM95).

Flow Cytometry of Human Nucleolar Ag on 293T cells. Black: cells alone, Grey: Isotype Control, Green: CF488-labeled human Nucleolar MAb (NM95).

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Nucleolar Mouse Monoclonal Antibody (NM95).

Formalin-fixed, paraffin-embedded human Tonsil stained with Nucleolar Mouse Monoclonal Antibody (NM95).

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CF488 Conjugate of Nucleolar Mouse Monoclonal Antibody (NM95).

Flow Cytometry of Human Nucleolar Ag on 293T cells. Black: cells alone, Grey: Isotype Control, Green: AF488-labeled human Nucleolar MAb (NM95).

SDS-PAGE Analysis Purified Nucleolar Mouse Monoclonal Antibody (NM95). Confirmation of Purity and Integrity of Antibody

Flow Cytometric Analysis of PFA-fixed K562 cells using Nucleolar Mouse Monoclonal Antibody (NM95) followed by Goat anti-Mouse IgG-CF488 (Blue), Isotype Control (Red)

Immunofluorescence Analysis of PFA-fixed K562 cells labeling Nucleolin with Nucleolar Mouse Monoclonal Antibody (NM95) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.

SDS-PAGE Analysis of Purified NM95 Mouse Monoclonal Antibody (NM95). Confirmation of Purity and Integrity of Antibody.