Recognizes an unidentified antigen in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell type in human tissues. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. MAb BM-2 reacts with early precursor and mature forms of human myeloid cells. This MAb is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes.
Clonality:
Monoclonal
Clone Designation:
[SPM250]
Molecular Weight:
Unknown
Isotype:
IgG1
NCBI:
Not Known
Form:
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
ELISA (For coating, order Ab without BSA), Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Immunohistochemistry (Frozen & Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (No special pretreatment is required for staining of formalin/p
Formalin-fixed, paraffin-embedded human Tonsil stained with Granulocyte Monoclonal Antibody (SPM250).
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