Hexa-histidine tagged human CD70 recombinant protein
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of hexa-histidine. This MAb is highly specific to hexa-histidine and shows no cross-reaction with other structurally related compounds. It has a very high affinity for hexa-histidine and is excellent for use in various techniques. His-Tag Antibody detects recombinant proteins containing the 6xHis epitope tag. The antibody recognizes the 6xHis-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein s biochemical properties. A variety of plasmids contain DNA that encodes an amino-terminal tag consisting of six histidine (6xHis) residues followed by an extended multiple cloning site. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography. As is the case with other protein tag systems, this polyhistidine tag can often be cleaved at sites recognized by proteases such as thrombin and enterokinases to isolate the protein of interest.
Clonality:
Monoclonal
Clone Designation:
[r6HIS/6423]
Molecular Weight:
244Da
Isotype:
IgG1
NCBI:
Not Applicable
Form:
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Application Notes:
Confocal Microscopy, Western Blot (1-2ug/ml),,Flow Cytometry (0.5-1ug/million cells), Immunofluorescence (0.5-1ug/ml), ,Immunoprecipitation (2-4ug/500ug proteins),,Optimal dilution for a specific application should be determined.
SDS-PAGE Analysis of Purified Anti-Hexa-histidine Recombinant Mouse Monoclonal r6HIS/6423). Confirmation of Integrity and Purity of Antibody.
Western blot analysis of recombinant His-Tag protein using Anti-Hexa-histidine Recombinant Mouse Monoclonal r6HIS/6423).
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