Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an alpha chain (36kDa) and a beta chain (27kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six alpha and ten beta chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooli
Formalin-fixed, paraffin-embedded human tonsil stained with HLA-Pan Mouse Monoclo
Formalin-fixed, paraffin-embedded human lymph node stained with HLA-Pan Mouse Monoclonal Antibody (HLA-Pan/6663). Inset: PBS instead of primary, secondary negative control.
Formalin-fixed, paraffin-embedded human tonsil stained with HLA-Pan Mouse Monoclonal Antibody (HLA-Pan/6663).
Western Blot Analysis of HDLM-2 cell lysate using Pan-HLA-II (DP, DQ, DR) Mouse Monoclonal Antibody (HLA-Pan/6663).
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