This MAb recognizes a 60kDa antigen associated with the mitochondria in cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the mitochondria in cells from a wide variety of animals, but not insects and bacteria. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
Clonality:
Monoclonal
Clone Designation:
[MTC719]
Molecular Weight:
60kDa
Isotype:
IgG1
NCBI:
Not Known
Form:
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (0.25-0.5ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with
Formalin-fixed, paraffin-
Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Mitochondria Monoclonal Antibody (MTC719)
Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Mitochondria Monoclonal Antibody (MTC719)
Formalin-fixed, paraffin-embedded human Pancreas stained with Mitochondria Monoclonal Antibody (MTC719).
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