This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
Clonality:
Monoclonal
Clone Designation:
[235-1]
Molecular Weight:
70kDa & 80kDa
Isotype:
IgG1
NCBI:
Not Known
Form:
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), ,Immunoprecipitation (1-2 g/500ug protein lysate),,Immunocytochemistry (Acetone-fixed cells) (1-2ug/ml for 30 minutes at RT),Immunohistology (Frozen) (1-2ug/ml for 30 minutes at RT),Opt
Flow Cytometry of Human Nuclear Ag (HNA) on 293T cells. Black: Cells alone, Grey: Isotype Control, Green: CF488-labeled HNA MAb (235-1).
Flow Cytometric analysis of Human Nuclear Antigen on MCF-7 cells. Black: cells alone, Green: Isotype Control, Red: PE-labeled HNA Monoclonal Antibody (235-1).
Flow Cytometric analysis of Human Nuclear Antigen on HeLa cells. Black: cells alone, Green: Isotype Control, Red: PE-labeled HNA Monoclonal Antibody (235-1).
SDS-PAGE Analysis Purified Human-Nuclear-Antigen Mouse Monoclonal Antibody (235-1). Confirmation of Purity and Integrity of Antibody.
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