Bovine, Frog, Goat, Guinea pig, Human, Monkey, Mouse, Porcine, Rat
Immunogen:
Keratin-enriched preparation from cultured human epithelial cells
Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59kDa (CK4), 58kDa (CK5), 56kDa (CK6), 52kDa (CK8), 56.5kDa (CK10), 53kDa (CK13) and 45kDa (CK18). This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Application Notes:
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes), Optimal dilution
SDS-PAGE Analysis of Purified Multi-Cytokeratin Mouse Recombinant Monoclonal Antibody (rKRT/457).
Formalin-fixed, paraffin-embedded human Skin stained with Multi-Cytokeratin Mouse Recombinant Monoclonal Antibody (rKRT/457).
SDS-PAGE Analysis of Purified Multi-Cytokeratin Mouse Recombinant Monoclonal Antibody (rKRT/457).
Western Blot Analysis of Human Skin, A431 and HeLa lysates using Cytokeratin, Multi Mouse Recombinant Monoclonal Antibody (rKRT/457).
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