Recognizes a disaccharide epitope, Gal 1-3GalNAc, of Thomsen-Friedenreich (TF) antigen. It is specific for both anomeric forms of the disaccharide (TF and TF , including related structures on the glycolipid) and shows no cross-reactivity with sialylated glycophorin. The TF antigen acts as an oncofetal antigen, with low expression in normal adult tissues but increasing to fetal levels of expression in hyperplasia or malignancy. Considered to be a pan-carcinoma marker. It is capable to agglutinate desialylated red blood cells. During metastasis, the ability of malignant cells to form multicellular aggregates via homotypic or heterotypic aggregation and their adhesion to the endothelium are critical. It is involved in tumor cell adhesion and tissue invasion. It also causes an immune response, and overexpression of the antigen causes cancer cells to be more sensitive to natural killer cell lysis. TF antigen is suppressed in normal healthy cells and represents one of the few chemically well-defined antigens associated with tumor malignancy. Its presence on the surface of cancer cells may result from a divergence from the normal pathway for O-linked glycosylation in these cells, most likely caused by inappropriate localization of the enzymes involved in synthesis of the disaccharide.
Clonality:
Monoclonal
Clone Designation:
[A84-A/F10]
Molecular Weight:
Multiple
Isotype:
IgM
NCBI:
Not Applicable
Form:
200ug/ml of Ab Purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
For
SDS-PAGE Analysis of Purified T-F Antigen Mouse Monoclonal Antibody (A84-A/F10). Confirmation of Purity and Integrity of Antibody.
Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with T-F Antigen Mouse Monoclonal Antibody (A84-A/F10).
Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with T-F Antigen Mouse Monoclonal Antibody (A84-A/F10).
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