Cytokeratin, Basic (Type II or HMW) (Epithelial Marker) Antibody, IgG1, Clone: [SPM116], Mouse, Monoclonal

Product Overview

• Article Name: Cytokeratin, Basic (Type II or HMW) (Epithelial Marker) Antibody, IgG1, Clone: [SPM116], Mouse, Monoclonal
• Biozol Catalog Number: NBT-MSM5X-257-P1ABX
• Supplier Catalog Number: MSM5X-257-P1ABX
• Alternative Catalog Number: NBT-MSM5X-257-P1ABX-100
• Manufacturer: NeoBiotechnologies
• Host: Mouse
• Category: Antikörper
• Application: FC, IF, IHC, WB
• Species Reactivity: Bovine, Canine, Gallus, Human, Monkey, Mouse, Rabbit, Rat
• Immunogen: Human epidermal keratin
• Alternative Names: KRT2B, KRT2P, HUMCYT2A, Keratin, type II Cytoskeletal 2 oral, K76, Keratin 2p (K2P), Keratin-76, Cytokeratin-2P (CK-2P, Type-II Keratin Kb9

Product Description

This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1), 64kDa (CK3), 59kDa (CK4), 58kDa (CK5), 56kDa (CK6), 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50, 48, 46, 45, and 40kDa. This MAb recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. SPM115/SPM116 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [SPM116]
• Molecular Weight: 67kDa (CK1), 64kDa (CK3), 59kDa (CK4), 58kDa (CK5), 56kDa (CK6), 52kDa (CK8)
• Isotype: IgG1
• NCBI: 51350
• UniProt: Q01546
• Form: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Monoclonal Antibody

Application Notes

• Application Notes: Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml f), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM ED

Images

Formalin-paraffin human Breast Carcinoma stained with Cytokeratin, HMW MAb (SPM116).

Formalin-paraffin human Breast Carcinoma stained with Cytokeratin, HMW MAb (SPM116).