Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) Antibody, IgG1, Clone: [KRTL/1077], Mouse, Monoclonal

Product Overview

• Article Name: Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) Antibody, IgG1, Clone: [KRTL/1077], Mouse, Monoclonal
• Biozol Catalog Number: NBT-MSM8-1077-P0
• Supplier Catalog Number: MSM8-1077-P0
• Alternative Catalog Number: NBT-MSM8-1077-P0-20,NBT-MSM8-1077-P0-100
• Manufacturer: NeoBiotechnologies
• Host: Mouse
• Category: Antikörper
• Application: IHC
• Species Reactivity: Human, Rat
• Immunogen: Recombinant human KRT77 protein
• Alternative Names: K1B, KRT1B, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B

Product Description

This MAb recognizes the 56.5kDa (CK10), 50kDa (CK14), 50kDa (CK15), 48kDa (CK16), 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50, 48, 46, 45, and 40kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [KRTL/1077]
• Molecular Weight: 56.5kDa (CK10), 50kDa (CK14), 50kDa (CK15), 48kDa (CK16), 40kDa (CK19)
• Isotype: IgG1
• NCBI: 374454
• UniProt: Q7Z794
• Form: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Monoclonal Antibody

Application Notes

• Application Notes: Immunohistochemistry (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes), Optimal dilution f

Images

Formalin-fixed, paraffin-embedded Rat

Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin, LMW Monoclonal Antibody (KRTL/1077).

Formalin-fixed, paraffin-embedded Rat Oviduct stained with Cytokeratin, HMW Monoclonal Antibody (KRTL/1077).

Formalin-fixed, paraffin-embedded Rat Stomach with Cytokeratin, HMW Monoclonal Antibody (KRTL/1077).