Recombinant Cytokeratin, pan (Epithelial Marker) Antibody, Clone: [KRTH/1576R + KRTL/1577R], Rabbit, Monoclonal

Product Overview

• Article Name: Recombinant Cytokeratin, pan (Epithelial Marker) Antibody, Clone: [KRTH/1576R + KRTL/1577R], Rabbit, Monoclonal
• Biozol Catalog Number: NBT-RBM23-1600-P1ABX
• Supplier Catalog Number: RBM23-1600-P1ABX
• Alternative Catalog Number: NBT-RBM23-1600-P1ABX-100
• Manufacturer: NeoBiotechnologies
• Host: Rabbit
• Category: Antikörper
• Application: IHC, WB
• Species Reactivity: Canine, Guinea pig, Human, Mouse, Rabbit, Rat
• Immunogen: Recombinant full-length human KRT76 and KRT77 proteins
• Alternative Names: K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39

Product Description

Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67kDa (CK1), 64kDa (CK3), 59kDa (CK4), 58kDa (CK5), 56kDa (CK6), 52kDa (CK8), 56.5kDa (CK10), 50kDa (CK14), 50kDa (CK15), 48kDa (CK16), 40kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. It is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It may be useful to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has high sensitivity in the recognition of epithelial cells and carcinomas.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [KRTH/1576R + KRTL/1577R]
• Molecular Weight: 40-67kDa
• NCBI: 374454
• UniProt: Q7Z794
• Form: 200ug/ml of recombinant MAb Purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Recombinant Monoclonal Antibody

Application Notes

• Application Notes: Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes), Optimal dilution

Images

Formalin-fixed, paraffin-embedded human Skin stained with Pan-Cytokeratin Recombinant Rabbit Monoclonal Antibody (KRTH/1576R + KRTL/1577R).

Western blot analysis of Human Skin, Mouse Skin, Rat Skin, Hamster Skin, Guinea pig Skin, and Rabbit Skin tissue lysates using Cytokeratin, pan Recombinant Rabbit Monoclonal Antibody (KRTH/1576R + KRTL/1577R).