Recombinant HLA Class 1 ABC (MHC I) Antibody, Clone: [MHC-I/8147R], Rabbit, Monoclonal

Product Overview

• Article Name: Recombinant HLA Class 1 ABC (MHC I) Antibody, Clone: [MHC-I/8147R], Rabbit, Monoclonal
• Biozol Catalog Number: NBT-RBM3-8147-P0
• Supplier Catalog Number: RBM3-8147-P0
• Alternative Catalog Number: NBT-RBM3-8147-P0-20,NBT-RBM3-8147-P0-100
• Manufacturer: NeoBiotechnologies
• Host: Rabbit
• Category: Antikörper
• Application: IHC, WB
• Species Reactivity: Human
• Immunogen: Recombinant fragment (around aa100-300) of human HLA-I protein (exact sequence is proprietary)
• Alternative Names: Antigen Presenting Molecule, Cw*1701, D6S204, FLJ27082, HLA A, HLA B, HLA C, HLA class 1 A, HLA class 1 B, HLA class 1 C, HLA JY3, HLAA, HLAB, HLAC, HLC C, Human Leucocyte Antigen C, Major Histocompatibility Complex Class I A, Major Histocompatibility Complex Class I B, Major Histocompatibility Complex Class I C, Major histocompatibility complex, class I, A + B + C, MHC class I antigen A*1, MHC Class I Antigen, MHC class I antigen B*7, MHC class I antigen Cw*1, MHC class I HLA A, MHC class I HLA B, MHC class I HLA C, MHC HLA ABC,

Product Description

Major Histocompatibility Complex (MHC) is a genetic region on chromosomes composed of tightly linked highly polymorphic gene loci that encode cell surface specific proteins, depending on the structure and function of genes and coding products. We divide MHC into class OO OO, class OO OO and class OO OO gene regions. Endogenous antigen presentation is presented by the major histocompatibility complex OO OO (MHC OO OO) pathway. Intracytoplasmic proteins are decomposed into peptides during protease degradation, which in turn are delivered by HSP41 and HSP90 proteins to TAP on the membrane. MHC OO OO is widely distributed on the surface of almost all nucleated cells, including spleen, lymph nodes and thymus lymphocytes in normal tissues. It is also expressed in skin, lung, kidney, liver and heart tissues. It is also widely expressed in tumor tissues. MHC OO OOmolecular typing is related to the matching of organ transplantation and bone marrow transplantation, as well as a variety of autoimmune diseases.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [MHC-I/8147R]
• Molecular Weight: 40/41kDa
• NCBI: 3105
• UniProt: P01889
• Form: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Recombinant Monoclonal Antibody

Application Notes

• Application Notes: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes),Optimal dilutio

Images

Formalin-fixed, paraffin-embedded human tonsil stained with HLA 1 ABC Recombinant Rabbit Monoclonal Antibody (MHC-I/8147R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human tonsil stained with HLA 1 ABC Recombinant Rabbit Monoclonal Antibody (MHC-I/8147R). Inset: PBS instead of primary antibody, secondary only negative control.

Western Blot Analysis of HOP-92, Tonsil, Lung and Hep G2 lysates using HLA Class 1 ABC Recombinant Rabbit Monoclonal Antibody (MHC-I/8147R).

Western Blot Analysis of Human Spleen, Mouse Spleen, Rat Spleen, Hamster Spleen, Guinea pig Spleen, Human Lung, Mouse Lung, Rat Lung, Hamster Lung and Guinea pig Lung tissue lysates using HLA Class 1 ABC Recombinant Rabbit Monoclonal Antibody (MHC-I/8147R).