Recombinant Cytokeratin, Multi (Epithelial Marker) Antibody, Clone: [KRT/1877R], Rabbit, Monoclonal

Product Overview

• Article Name: Recombinant Cytokeratin, Multi (Epithelial Marker) Antibody, Clone: [KRT/1877R], Rabbit, Monoclonal
• Biozol Catalog Number: NBT-RBM37-1877-P0
• Supplier Catalog Number: RBM37-1877-P0
• Alternative Catalog Number: NBT-RBM37-1877-P0-20,NBT-RBM37-1877-P0-100
• Manufacturer: NeoBiotechnologies
• Host: Rabbit
• Category: Antikörper
• Application: FC, IF, IHC, WB
• Species Reactivity: Bovine, Frog, Goat, Guinea pig, Human, Monkey, Mouse, Porcine, Rat
• Immunogen: Keratin-enriched preparation from cultured human epithelial cells

Product Description

Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59kDa (CK4), 58kDa (CK5), 56kDa (CK6), 52kDa (CK8), 56.5kDa (CK10), 53kDa (CK13) and 45kDa (CK18). This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [KRT/1877R]
• Molecular Weight: Multiple
• NCBI: 3851
• UniProt: P02538
• Form: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Recombinant Monoclonal Antibody

Application Notes

• Application Notes: Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA

Images

Formalin-fixed, paraffin-embedded human Skin stained with Multi-Cytokeratin Recombinant Rabbit Monoclonal Antibody (KRT/1877R).

SDS-PAGE Analysis of Purified Multi-Cytokeratin Rabbit Monoclonal Antibody (KRT/1877R). Confirmation of Integrity and Purity of Antibody.

Immunofluorescence Analysis of MeOH-fixed Human MCF-7 cells labeling CK with Multi-Cytokeratin Recombinant Rabbit Monoclonal Antibody (KRT/1877R)followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

Western Blot Analysis of A431 cell lysate using Cytokeratin Recombinant Rabbit Monoclonal Antibody (KRT/1877R).