Recombinant Cytokeratin, pan (Epithelial Marker) Antibody, Clone: [Cocktail PCK/4933R], Rabbit, Monoclonal

Product Overview

• Article Name: Recombinant Cytokeratin, pan (Epithelial Marker) Antibody, Clone: [Cocktail PCK/4933R], Rabbit, Monoclonal
• Biozol Catalog Number: NBT-RBM43-4933-P1ABX
• Supplier Catalog Number: RBM43-4933-P1ABX
• Alternative Catalog Number: NBT-RBM43-4933-P1ABX-100
• Manufacturer: NeoBiotechnologies
• Host: Rabbit
• Category: Antikörper
• Application: IHC, WB
• Species Reactivity: Human
• Immunogen: Crude cytokeratin extract prepared from RT-4 and MCF-7 cells
• Alternative Names: KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39

Product Description

PCK/4933R demonstrates a broad spectrum of cytokeratin reactivity. In normal tissues, PCK/4933R is reactive with most epithelial types, including bile ducts and hepatocytes in liver, bladder epithelium, breast ducts, bronchial epithelium, endometrium, intestinal epithelium of stomach, duodenum, ileum, colon, rectum, pancreas, ovarian epithelium, pancreatic acini, pituitary acini, pneumocytes, prostate, thyroid, skin (positive on the basal layer and negative on the superficial layers of squamous epithelium), and apocrine and sweat glands. In tumors, PCK/4933R is reactive with most carcinomas, including breast, transitional cell (TCC), renal cell (RCC), lung adenocarcinoma, lung small cell, lung squamous cell, endometrial, prostate, ovarian, hepatocellular (HCC), colorectal CA, stomach and thyroid. It is negative in certain normal tissues, including brain, lymphocytes and all cells of hematolymphoid origin, muscle, brain, nerves, endothelium and in certain tumors including most melanomas, sarcomas, lymphomas, primitive neuroectodermal tumors (PNET)/Ewings and gastrointestinal stromal tumors (GIST). Positivity has been seen on some dendritic cells in lymph nodes, some endothelia, and some muscle cells.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [Cocktail PCK/4933R]
• Molecular Weight: 40-67kDa (Multiple)
• NCBI: Multiple
• Form: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Recombinant Monoclonal Antibody

Application Notes

• Application Notes: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal diluti

Images

Formalin-fixed, paraffin-embedded human prostate stained with Pan-Cytokeratin Recombinant Rabbit Monoclonal Antibody (PCK/4933R). Inset: PBS instead of primary antibody, secondary control.

Western Blot Analysis of Human skin and HeLa lysates using Cytokeratin, pan Recombinant Rabbit Monoclonal Antibody (PCK/4933R).