MECA-79 Antibody, IgM, Clone: [MECA-79], Rat, Monoclonal
Biozol Catalog Number:
NBT-RTM1-1599-P1BX
Supplier Catalog Number:
RTM1-1599-P1BX
Alternative Catalog Number:
NBT-RTM1-1599-P1BX-100
Manufacturer:
NeoBiotechnologies
Host:
Rat
Category:
Antikörper
Application:
ELISA, FC, IF, IHC, WB
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Mouse lymph node stromal cells
Alternative Names:
MECA-79, Peripheral Node Addressin (PNAd)
MECA-79 recognizes a carbohydrate epitope shared with a group of sulfation decorated sialomucins, including sulfated ligands for CD62L (CD34, GlyCAM-1, Sgp200, and a subset of MAdCAM-1). This set antigens has been referred to as peripheral node addressin (PNAd) with the molecular mass 50-250 kD. It has been identified that GlcNAc-6-SO4 sulfation contributes to MECA-79 binding and the core 1 beta1,3-N-acetylglucosaminyltransferase is required for the generation of the MECA-79 epitope. MECA-79 is expressed on high endothelial venules (HEV) of lymphoid tissues, chronic inflamed tissues and rheumatoid synovia. The interaction of PNAd with CD62L receptor is involved in tethering and rolling of lymphocytes along HEV in lymphoid tissues. MECA-79 antibody reacts with Mouse, human and many other species PNAd and blocks L-selectin-dependent lymphocyte adhesion in vitro and in vivo.
Clonality:
Monoclonal
Clone Designation:
[MECA-79]
Molecular Weight:
90-110kDa
Isotype:
IgM
NCBI:
Not Known
Form:
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
ELISA (For coating, order antibody without BSA), Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requir
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