Mouse anti Human CD2, IgG2a, Clone: [T6.3], Unconjugated, Monoclonal

Product Overview

• Article Name: Mouse anti Human CD2, IgG2a, Clone: [T6.3], Unconjugated, Monoclonal
• Biozol Catalog Number: NMB-0021
• Supplier Catalog Number: 0021
• Alternative Catalog Number: NMB-0021
• Manufacturer: NordicMubio
• Host: Mouse
• Category: Antikörper
• Application: FC
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: T-cell surface antigen CD2, CD2 cytoplasmic domain-binding protein

Product Description

Mouse anti human CD2 can be used for identification of human T cells and a subset of NK cells associated with the receptor for sheep erythocytes rosettes expressing the 45-50,000 M.W. surface antigen.

Product Properties

• Clonality: Monoclonal
• Concentration: See vial for concentration
• Clone Designation: [T6.3]
• Isotype: IgG2a
• UniProt: P06729
• Buffer: Provided as solution in phosphate buffered saline with 0.08% sodium azide
• Source: Derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with T Lymphocytes activated by a mixed lymphocyte culture.
• Purity: Protein A/G Chromatography
• Form: Unconjugated
• Formula: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Application Notes

• Application Notes: Flow cytometry: PBMC: Add 10 µl of antibody to 1 million PBMCs in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8oC. Wash twice with PBS, incubate with an appropriate concentratio with a fluorochrome conjugated secondary antibody, wash again and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of antibody to 100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20oC. Lyse the whole blood. Wash once with PBS, incubate with an appropriate concentratio with a fluorochrome conjugated secondary antibody, wash again and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for immunofluorescence analysis with a flow cytometer or microscope.