Mouse anti Human CD4, IgG1, Clone: [7E14], Unconjugated, Monoclonal

Product Overview

• Article Name: Mouse anti Human CD4, IgG1, Clone: [7E14], Unconjugated, Monoclonal
• Biozol Catalog Number: NMB-0041
• Supplier Catalog Number: 0041
• Alternative Catalog Number: NMB-0041
• Manufacturer: NordicMubio
• Host: Mouse
• Category: Antikörper
• Application: FC
• Species Reactivity: Human
• Immunogen: Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.
• Conjugation: Unconjugated
• Alternative Names: T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3

Product Description

Identification of human helper/inducer T cells expressing the 60,000 M.W. surface antigen (HLA class II reactive). CD4 is present in low density on monocytes.

Product Properties

• Clonality: Monoclonal
• Concentration: See vial for concentration
• Clone Designation: [7E14]
• Isotype: IgG1
• UniProt: P01730
• Buffer: Provided as solution in phosphate buffered saline with 0.08% sodium azide
• Purity: Protein A/G Chromatography
• Form: Unconjugated
• Formula: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Application Notes

• Application Notes: PBMC: Add10 µl of MAB/10 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2to 8oC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20oC. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5 +: Excites at 488nm and emits at 670nm. Store protected from light.