Mouse IgG2a FITC Negative Control
Catalog Number:
NMB-02A2
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| Article Name: | Mouse IgG2a FITC Negative Control |
| Biozol Catalog Number: | NMB-02A2 |
| Supplier Catalog Number: | 02A2 |
| Alternative Catalog Number: | NMB-02A2 |
| Manufacturer: | NordicMubio |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | FC |
| Conjugation: | FITC |
| Negative control for immunofluorescence staining with FITC conjugated mouse monoclonal antibodies. Assessment of non-specific binding of mouse monoclonal antibodies to human cell surface antigens. |
| Concentration: | See vial for concentration |
| Isotype: | IgG2a |
| Buffer: | Purified mouse IgG2a conjugated to FITC, as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein |
| Purity: | Protein A/G Chromatography |
| Formula: | Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein |
| Application Notes: | Consult the appropriate fact sheet to determine the amount of antibodies to be used as a control for PBMC or Whole Blood. PBMC: Add10 µl of MAB/10 6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20C. Lyse the whole blood. Wash once with PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. |
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