Mouse anti Human CD45, IgG1, Clone: [BHPT-1], Unconjugated, Monoclonal

Product Overview

• Article Name: Mouse anti Human CD45, IgG1, Clone: [BHPT-1], Unconjugated, Monoclonal
• Biozol Catalog Number: NMB-0451
• Supplier Catalog Number: 0451
• Alternative Catalog Number: NMB-0451
• Manufacturer: NordicMubio
• Host: Mouse
• Category: Antikörper
• Application: FC
• Species Reactivity: Human
• Immunogen: Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells of BALB/c mice immunized with peripheral blood monocytes from rheumatoid arthritis patient
• Conjugation: Unconjugated
• Alternative Names: CD45

Product Description

Identification of all human leukocytes, lymphocytes, monocytes, polymorphonuclear cells, eosinophils and basophils in peripheral blood expressing the 180-220 kD M. W. T200 family surface antigen. CD45 is also present on thymus, spleen, tonsil and progeni

Product Properties

• Clonality: Monoclonal
• Concentration: See vial for concentration
• Clone Designation: [BHPT-1]
• Isotype: IgG1
• UniProt: P08575
• Buffer: Provided as solution in phosphate buffered saline with 0.08% sodium azide
• Purity: Protein A/G Chromatography
• Form: Unconjugated
• Formula: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Application Notes

• Application Notes: PBMC: Add 10 µl of MAB/10 6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5+: As above, however use only 5 µl per test rather than 10 µl. Excites at 488nm and emits at 670nm. Store protected from light.