Mouse anti Human CD54, IgG2b, Clone: [D3.6], Unconjugated, Monoclonal

Product Overview

• Article Name: Mouse anti Human CD54, IgG2b, Clone: [D3.6], Unconjugated, Monoclonal
• Biozol Catalog Number: NMB-0541
• Supplier Catalog Number: 0541
• Alternative Catalog Number: NMB-0541
• Manufacturer: NordicMubio
• Host: Mouse
• Category: Antikörper
• Application: FC
• Species Reactivity: Human
• Immunogen: CD54=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with the major histocompatibility complex (MHC) class II-negative human b-lymphoblastoid cell line 6.16c13
• Conjugation: Unconjugated
• Alternative Names: CD54

Product Description

CD54, M.W. 90,000, antigen is the Intercellular Adhesion Molecule-1 (ICAM-1) and is the ligand for LFA-1 mediated adhesion. CD54 is weakly expressed on peripheral blood leukocytes. Upon activation the antigen is expressed strongly on B cells, T cells, ma

Product Properties

• Clonality: Monoclonal
• Concentration: See vial for concentration
• Clone Designation: [D3.6]
• Isotype: IgG2b
• UniProt: P05362
• Buffer: Provided as solution in phosphate buffered saline with 0.08% sodium azide
• Purity: Protein A/G Chromatography
• Form: Unconjugated
• Formula: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Application Notes

• Application Notes: PBMC: Add10 µl of MAB/10 6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8 C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20 C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.