Mouse anti double-stranded RNA (J2, J5 and K1) Comparison Set, Clone: [J2 J5 and K1], Monoclonal
Biozol Catalog Number:
NMB-10050100
Supplier Catalog Number:
10050100
Alternative Catalog Number:
NMB-10050100
Manufacturer:
NordicMubio
Host:
Mouse
Category:
Antikörper
Application:
DOT, ELISA, IAC, ICC, IHC
Alternative Names:
Anti-dsRNA mAb Comparison Set
Over the past decade our double-stranded RNA (dsRNA)antibodies have been used extensively to detect and characterise plant and animal viruses with dsRNA genomes or intermediates. In addition, the anti-dsRNA antibodies can be used as a diagnostic tool to
Clonality:
Monoclonal
Concentration:
Concentration after reconstitution: 1.00 mg/ml as determined by A280 nm (A280 nm = 1.47 corresponds to 1 mg/ml antibody).
Clone Designation:
[J2 J5 and K1]
Isotype:
IgG2a, IgG2b, IgG2a
Buffer:
Mouse monoclonal antibody J2 recognises double-stranded RNA (dsRNA) provided that the length of the helix is greater than or equal to 40 bp. dsRNA-recognition is independent of the sequence and nucleotide composition of the antigen. All naturally occurrin
Source:
Female DBA/2 mice were injected intraperitonially with a mixture of 50 ug L-dsRNA and 75 ug methylated bovine serum albumin, emulsified in complete Freunds adjuvant. After several boosts spleen cells were fused with Sp2/0-Agl4 myeloma cells to generate th
Purity:
Gel electrophoretically pure IgG antibody.
Formula:
The lyophilised samples should each be reconstituted with 100 µl sterile distilled water. The mAb will then be in PBS without any stabilisers or preservatives at a concentration of 1 mgr/ml. As a result of the lyophilisation procedure, the reconstituted
Application Notes:
Mouse monoclonal antibodies J2, J5 and K1 can be used for ELISA, dsRNA-immunoblotting, immunoaffinity chromatography and in certain systems also for immunohistochemistry (see references). The optimum working dilution of each antibody for any specific application should be established by titration. Please note that nucleic acid separation prior to dsRNA-immunoblotting must be carried out by polyacrylamide gel electrophoresis, because the sensitivity of detection is considerably lower after blotting from agarose gels. Not for use for clinical purposes. For in vitro use only.
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