Egg-yolk derived affinity purified IgY in phosphate buffered saline, pH 7.2 with 0.075% sodium azide
Source:
Chickens were immunized with highly purified Toxin B purified from Clostridium difficile. After multiple immunizations in Freunds adjuvant, eggs were collected and the IgY purified. Anti-Clostridium difficile Toxin B was immunoaffinity purified using the
Form:
Affinity purified
Application Notes:
This antibody is suitable for ELISA and Western Blotting procedures. By Direct ELISA, the half-point titre was 1:20,000 using a 2 ug/ml coating of Toxin B. This antibody has also been successfully used in cytotoxicity and neutralization assays (please see embedded image). Optimal working dilutions should be determined for your particular assay conditions.
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