Chickens were immunized with FLAG Epitope (DYKDDDDK) conjugated to KLH. After multiple immunizations, eggs were collected and the immunoglobulin fraction prepared. Antibodies were immunoaffinity purified using the peptide immobilized on a solid support.
Form:
Affinity purified
Formula:
Phosphate buffered saline, pH 7.2 with 0.09% sodium azide
Application Notes:
Antibody was analyzed using amino-terminal Met-Flag-BAP, amino- terminal FLAG-BAP and carboxy-terminal FLAG-BAP fusion proteins and Invitrogen Positope R900-40. ELISA: We suggest a 1:5,000-1:10,000 dilution. WESTERN BLOTS: We suggest a 1:2,000 to 1:10,000 dilution. For immunohistochemistry, we suggest a 1:500 dilution (see image). Please note that the investigator must determine the appropriate dilution for your particular application.
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