Mouse anti Human CD3 FITC - CD44 PE, FITC/RPE

Product Overview

• Article Name: Mouse anti Human CD3 FITC - CD44 PE, FITC/RPE
• Biozol Catalog Number: NMB-B344
• Supplier Catalog Number: B344
• Alternative Catalog Number: NMB-B344
• Manufacturer: NordicMubio
• Host: Mouse
• Category: Antikörper
• Application: FC
• Species Reactivity: Human
• Immunogen: CD3=Derived from the hybridization of mouse NS-1 myeloma celss with spleen cells from BALB/c mice immunized with human thymocytes. CD44=Derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with TCR-gamma epsilon thymocytes
• Conjugation: FITC/RPE
• Alternative Names: CD3 FITC - CD44 PE

Product Description

The CD3 epitope is expressed on the epsilon chain of the CD3/T cell antigen receptor (TcR) complex. CD3 is present on 65-85% of thymocytes and has a mitogenic effect on peripheral blood T cells. Indentification of human T cells expressing the 22-28,000 M

Product Properties

• Concentration: Titered for flow cytometry
• UniProt: P07766
• Buffer: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
• Purity: Protein A/G Chromatography
• Form: Bi-Test (FITC/RPE) Reagent
• Formula: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein

Application Notes

• Application Notes: PBMC: Add10 µl of MAB/10 6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.