CD4=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.
Conjugation:
FITC/RPE
Alternative Names:
CD4 FITC - HLADR PE
CD4 identifies human helper/inducer T cells expressing the 60,000 M.W. surface antigen (HLA class II reactive). CD4 is present in low density on monocytes. HLA-DR identifies human B cell and T cell subsets associated with approximately 10% of peripheral
Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
Purity:
Protein A/G Chromatography
Form:
Bi-Test (FITC/RPE) Reagent
Formula:
Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
Application Notes:
PBMC: Add10 µl of MAB/10 6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20C. Lyse the whole blood. Wash once with PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
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