IgG1 Negative Control, conjugated to PE, Clone: [VI-AP], Mouse, Monoclonal
Biozol Catalog Number:
NMB-GM-4993
Supplier Catalog Number:
GM-4993
Alternative Catalog Number:
NMB-GM-4993
Manufacturer:
NordicMubio
Host:
Mouse
Category:
Antikörper
Application:
FC, IF
Conjugation:
PE
This ready to use Negative Control reagent contains purified, fluorescein or phycoerythrin conjugated mouse immunoglobulin molecules of IgG1 isotype, which have been selected on the basis of their binding characteristics: no specific binding to human cel
Clonality:
Monoclonal
Clone Designation:
[VI-AP]
Isotype:
IgG1
Buffer:
2ml of PE-conjugated VI-AP in PBS pH 7.2, 1% BSA, and 0.05% NaN3, approximately 100 tests.
Form:
PE
Formula:
PBS pH 7.2, 1% BSA, 0.05% NaN3
Application Notes:
Direct Immunofluorescence (Staining Procedure) Nordic-MUbio fluorochrome labeled antibodies are designed for use with either whole blood or isolated mononuclear cell (MNC) preparations. Proposed staining procedure for whole blood in short: - For each sample add 50 µl of EDTA anti-coagulated blood to a 3-5 ml tube - Add 20 µl of the appropriate Nordic-MUbio monoclonal antibody conjugate - Incubate the tube for 15 minutes at 4C or at room temperature in the dark - Add 100 µl NM-LYSE (Cat.No. GAS-003) to each tube and incubate for 10 minutes at room temperature - Add 3-4 ml of destilled water and vortex, incubate for 5-10 minutes at room temperature - Centrifuge tube for 5 minutes at 300 g - Aspirate supernatant and resuspend pellet in 0.3 ml of sheath fluid - Analyze immediately or store samples at 2-8 C in the dark and analyze within 24 hours For ''No-Wash protocol please refer to www.nordicmubio.com Proposed staining procedure for MNC in short: - Carefully add 20 µl antibody conjugate and 50-100 µl MNC to the bottom of a tube - Vortex at low speed for 1-2 seconds - Incubate for 15-30 minutes at 2-8C or at room temperature - Centrifuge tubes for 5 minutes at 300 g - Remove supernatant, resuspend cells in 2-5 ml of phosphate buffered saline (PBS) and centrifuge cells again for 5 minutes at 300 g - Remove supernatant and resuspend cells in sheath fluid for immediate analysis or resuspend cells in 0.5 ml 1 % formaldehyde and store them at 2-8C in the dark. Analyze fixed cells within 24 hours
GM-4993
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